Talinum paniculatum
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Transcript of Talinum paniculatum
NATASHA KAUR
AMARPREET KAUR
YAASHINI
SELVARAJAH
KRISNAVIMALA
KRISHUAN
NABILAH BINTI
YUSOF
NORIZA BINTI
ISHAK
AMIRAH BINTI
SHAARI
HILDA ANAK EMAK
AIN NAJWA BINTI
AMRAN
NURUL HIDAYAH
BINTI MANSOR
ONG SHWU CHYN
PUI WAN TING
WONG SIEW CHING
Five Mus musculus have been used to investigate the effect of
(ginseng jawa) to the liver, kidney and intestine within `
of experimental.
• Class of mammalian
• Easy to get
• Easy to handle-small in size
To know the effect of TalinumPaniculatum
size and
body weight of Mus musculus
Behaviour investigation of the
Mus musculus.
Talinum Paniculatum contains
vitamin c and
toxic
Tanin,
Saponin and
Flavoid.
So we carry out this experiment to see the side effects of Tanilum Paniculatum to the internal organs (intestine, kidney and liver) of Mus musculus.
Experiment applicable only to five Mus musculus.
only three leaves of TanilumPaniculatum used everyday.
Time limitation to only 20 days
DAVID FAIRCHILD,
COCONUT GROOVE,
FLORIDA
DR J.L HEID said that in his report :
In the green leaves of Talinum The Vitamin C
is about 160 mg per 100 gram
The Talinum show the phenomenally high content of vitamin C compared to guavas and sweet peppers.
It also probably has a considerable contents of carotenoid pigments.
Chemical content in Talinum paniculatumcomposed of
saponin,
flavanoid and
tanin.
Saponin - strengthen our body and
- improve blood circulation.
Saponin : can cause irritation in mucosa membrane, and can damage red blood cell through hemolysis
The root can be used to treat :
tiredness,
dizzy,
diarrhea,
aphrodisiacs and
pneumonia.
The leaves can be used to treat bump.
Also increase the sexual desire in male mencit.
5 mice (Mus musculus)
Bedding
3 ginseng jawa (Talinum paniculatum) leaves (0.5g) mix with 100 ml water
Mice food
Mice is weighed daily
Amount of water consumed are measured
Glove
Cotton and chloroform
Dissecting set
Dissecting tray and pins
Water
Beaker
Water was applied on the ventral side of mice
The kidney, small intestine and liver were dissected.
Fixation.
Dehydration.
Clearing.
Embedding.
Sectioning.
Coloring/ staining.
Fixation
Bouin’s fluid (<24 hours)
Bottles
Dehydration
Ethanol 70% (< 24 hours)
Ethanol 80%, 95%, 100% (< 1 hour)
Clearing
Toluene solution (< 24 hours)
Embedding (Paraffin processing) Paraffin solution (60C) (< 1 hour)
Paraffin solution (60C) (< 1 hour)
• (twice procedure)
Paraffin solution added in mold followed by the organ.
Tissue sample then was left for 5 days.
The infiltrating medium (paraffin) provides cellular support for cutting tissue sections.
materials methods Diamond pen
Distilled water
Albumin
Slide and slide cover
Microtome
Brush
The slices was placed on the slide.
Few drops of distilled water/albumin are dropped on the slides.
Intestine-distilled water
Albumin - liver and kidney
Heating (30C)
Materials Methods Xylen 1 And 2
Alcohol+xylen (1:1)
Alcohol, Alcohol( 90%,80%,75%)
Distilled Water
Hematoksilin Solution
0.5 % Sodium Bicarbonate Solution
Running Water
Scott’s Solution
Eosin
Color stain used :
• Harris Hematoksilin- Eosin coloring.
• Soaking of slides-
• into 21 piece of jar containing different types of chemicals and liquid
• based on the duration given.
• Gluing: DPX / Canada balsam
• Heating of slides
• (37 C)
• 1 week
0
5
10
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20
25
30
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Result
1
2
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4
5
Graph showing the increasing weight mass of the mice in 20 days
The weight of each mice
increase day by day
After taking Talinum paniculatum as their drink.
The Talinum paniculatum
rich with vitamin C
increases appetite
Gaining of body weight
Graph showing the amount of water and Talinum paniculatum consumed by the mice's
The goblet cell in this intestine secretes more mucous compared to the normal
intestine
40x magnification
Goblet cell
Wide spaces in between cells showing the sinusoid. This means that more enzymes
are secreted to detoxify the drug (ginseng jawa). In the normal slide the sinusoid is
compact.
40 x magnification
The presence of vitamin C in Talinumpaniculatum causes more enzyme to be released to detoxify the drugs in the liver cells.
prove: white spaces (sinusoid)seen in effected liver cells while the original cell the (sinusoid) is more compact.
The glomerular is tightly packed due to the toxic caused by the drug that is being
detoxify by the glomerular as compared to the normal kidney slide.
40x magnification
• act as rinsing agent to remove the blood from the organs, and its sterileNormal saline solution
• a fixing & preserving solution for the organsBouin’s solution-
• For dehydration processEthanol 70%,80%,95% & 100%
• used for the clearance purposeToluene
• to hold the organ tissues tightly during the sectioning process
paraffin
• Only the small intestine cell can withstand with the pressure.Distilled water
• to maintain the shape of the kidney and liver cell.Albumin
Effect of Talinum
paniculatum
Increase the appetite
Improve blood
circulation
Stimulate testosterone hormone to
enhance sexual desire
they will get attracted to mate becomes active and aggressive
Weight gain
Tannins precipitate proteins inhibits in some ruminant animals
for the absorption of nutrients from high-tannin grains
such as sorghum. Tannic acid does not affect absorption
of other trace minerals such as zinc, copper, and manganese in rats
Prove: the glomerulus at kidney cell is tightly packed compared to the original slide of kidney where there are some spaces between the glomerulus.
The lethal dose of Talinum paniculatum around 6% is able to increase the animal's body weight.
Prove: the mice’s body weight increases drastically in that 20 days.
Damage of the weighing machine.
Damage of Microscope.
Thank you..