COMUNICAÇÃO TÉCNICA · 2020. 7. 8. · Palestra apresentada no Clínica Mattos Pimenta de...
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Transcript of COMUNICAÇÃO TÉCNICA · 2020. 7. 8. · Palestra apresentada no Clínica Mattos Pimenta de...
COMUNICAÇÃO TÉCNICA ______________________________________________________________________________________________________________________________________________________________________________________________________
Nº 176819
Nanotechnology for tissue regeneration and nanodelivery Maria Helena Ambrosio Zanin
Palestra apresentada no Clínica Mattos Pimenta de Neurologia e Neurocirurgia Ltda,SãoPaulo, 2020.
A série “Comunicação Técnica” compreende trabalhos elaborados por técnicos do IPT, apresentados em eventos, publicados em revistas especializadas ou quando seu conteúdo apresentar relevância pública. ___________________________________________________________________________________________________
Instituto de Pesquisas Tecnológicas do Estado de São Paulo S/A - IPT
Av. Prof. Almeida Prado, 532 | Cidade Universitária ou Caixa Postal 0141 | CEP 01064-970
São Paulo | SP | Brasil | CEP 05508-901 Tel 11 3767 4374/4000 | Fax 11 3767-4099
www.ipt.br
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NANOTECHNOLOGY FOR TISSUE REGENERATION AND NANODELIVERY
Dra. Maria Helena Ambrosio Zanin
20/05/2020
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LPP – Chemical Processes and Particle Technology Laboratory Nanotechnology and advanced chemical processes LBI – Industrial Biotechnology Laboratory Development and characterization of biotecnolology solutions LMI - Micromanufacturing Laboratory Product and process miniaturization
Nucleus for Bionanomanufacturing
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Nanotechnology in Medicine - Application
• Drug delivery
• In vitro diagnostics
• In vivo imaging
• Therapy techniques
• Biomaterials
• Tissue engineering
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Biomaterials relies on four main classes of materials: i. Polymers, ii. Ceramics, iii. Metals iv. Composites (blends and combinations of the aforementioned materials). Biomaterials : natural or synthetic sources
Biomaterials applied in scaffold building
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Polymers
Natural Polymeric
Synthetic Polymeric
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Degradable periods of synthetic polymer
Materials Degradation
period
Hidrophobicity
Polycaprolactone (PCL) > 20 months Hydrophobic
Poly L-lactic acid (PLLA) 20 ≈ 60 months Hydrophobic
Polydioxanone (PDO) 6 months Hydrophilic
Polyglycolic acid (PGA) 1 ≈ 4 months Hydrophilic
Poly (lactic-co-glycolic acid) (PLGA) 2 months Depend of PLA and PGA compositions.
Increase of PLA => less hydrophilicity
Walker et.al. 2009.
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Nanofiber developed by Electrospinning
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Surface modification technique of electrospun nanofibers
A
Electrospun nanofibers Functionalized Surface
Plasma or wet chemical treatment
Biocompatible nanofibers
Biologically or therapeutically functionalized
nanofibers
B
Electrospun nanofibers Monomer
Induced by plasma or radiation
Surface graft polymerization
Immobilization of protein, enzyme,
growth factor, drug
Biologically or therapeutically functionalized
nanofibers
Blend solution
C
Electrospinning Surface orientation
Biologically or therapeutically
functional agents
D
Coaxial Electrospinning
Biologically or therapeutically
functional agents
Shell polymer
Core polymer
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PCL
PCL
Gelatin
Case studies of nanofiber Scaffold at IPT
Mechanical tensile strength
Vanessa Tiemi Kimura (MSc.) Supervisors: Dra.Wang Hui (EPUSP) /
Dra. Maria Helena Ambrosio Zanin (IPT) Wettability
Stem Cell
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GO
PLGA
NCTC-929
Cytotoxicity
Case studies of Scaffold at IPT ZANIN, M. H. A.; KIMURA, V. T. ; SANT'ANNA, D. A. .
Fabrication of Graphene oxide-PLGA hybrid nanofiber for
tissue engineering application'. In: 27th Assembly of
Advanced Materials Congress, 2019, Stockholm. Abstracts.... Stockholm: IAAM, 2019. p. 186-187.
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Aligned nanofiber scaffold for cardiomyocytes based on induced pluripotent stem cells
CellTracker green/Nuc Dead red/Hoechst wout nanof.
CellTracker green/Nuc Dead red/Hoechst PCL nanof.
TNNT2/TNNI3/Hoechst PCL nanof.
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Dra. Maria Helena Ambrosio Zanin
