Simpósio ABC/ANM

Produção de Vacinas no Brasil: Problemas,

Perspectivas e Desafios Estratégicos

A Imunologia e os Desafios para o

Desenvolvimento Racional de Vacinas

Marcello A. Barcinski (IOC-Fiocruz) Dezembro 2013

Cited in: Reverse Vaccinology: Developing

Vaccines in the Era of Genomics

Alessandro Sette and Rino Rappuoli

Immunity,33: 2010

Pasteur proposed that in order to make a

vaccine, one should ‘‘isolate, inactivate and

inject the microorganism”

The vaccines developed using Pasteur’s rules

became powerful tools in the history of medicine

and, in less than a century, led to the elimination

of some of the most devastating infectious

diseases globally.

At the end of the 20th century, most of the

vaccines that could be developed by these

traditional technologies had been developed,

and new technologies were required to conquer

the remaining pathogens.

Basic Rules of Vaccinology (Pasteur, 1880)

1. Doenças complexas em sua patogenicidade;

2. Agentes etiológicos exibem intensa

variabilidade

3. Agentes etiológicos desenvolveram

mecanismos eficientes de escape da

resposta imune do hospedeiro

Vacinologia X Imunologia

Desenvolvimento de Fármacos X Farmacologia?

Medicina Translacional ou Experimental?

Vacinologia X Imunologia

Expectativas em relação à vacinologia para o desenvolvimento

de vacinas de uma nova geração:

1. Caracterização dos mecanismos de imunoproteção e como mensura-los

2. Definição dos mecanismos de escape da resposta imune do hospedeiro;

3. Definição de estratégias para a indução de respostas imunoprotetoras.

Coffman et al. Immunology 33, 2010

Sette & Rappuoli, Immunity 33,530,2010

Vacinologia Reversa

Sette & Rappuoli, Immunity 33,530,2010

Pulendran B et al. Immunity,33:2010

Biologia dos Sistemas

Pulendran,B et al Immunity, 33, 2010

Pulendran B et al. Immunity,33:2010

Pulendran B et al. Immunity,33:2010

Pulendran B et al. Immunity,33:2010

Model of a candidate HIV vaccine prime immunogen (center) engaging germline B cell receptors (bottom) to initiate an antibody immune response.

Image: Christina Corbaci, Adam Gardner, Joe Jardine, Sergey Menis, and William Schief, The Scripps Research Institute

Anticorpos neutralizantes de amplo espectro (bNAbs)

Anticorpos neutralizantes de amplo espectro (bNAb)

J.Cohen,

Burton et al

Burton et al.

F. Klein et al. Science,341:1199,2013

F. Klein et al. Science,341:1199,2013

Anti-gp140 antibody cloning.

JF Scheid et al. Nature 000, 1-5 (2009) doi:10.1038/nature07930

To examine the memory antibody response to HIV, Scheid et al. cloned more than 500 antibodies from HIV-specific memory B cells from six HIV-infected patients with high serum titres of broadly neutralizing antibodies.

F. Klein et al. Science,341:1199,2013

F. Klein et al. Science,341:1199,2013