! "#$!

4- Descrição e avaliação do apoio institucional recebido no período

O Instituto de Biociências/UNESP contribuiu de forma significativa com as

atividades de pesquisa desenvolvidas relacionadas ao projeto em questão, fornecendo

materiais básicos para as atividades de rotina do laboratório como papel toalha, papel

higiênico, papel alumínio, filmes de pcv, lâminas e lamínulas histológicas, etanol,

formol, ácido acético, entre outros. Alguns itens de papelaria como canetas, fitas

adesivas, papel A4, também foram fornecidos pela UNESP. Além disso foi possível

contar também com apoio do setor de serviços do Instituto de Biociências na execução

de adequações na parte elétrica e hidráulica do laboratório Genômica Integrativa. A

execução do projeto contou ainda com dois técnicos laboratoriais e uma secretária que

prestaram serviços diretos às atividades de rotina do laboratório.

5- Recursos recebidos e aplicados na execução do projeto

Projeto 1: Elucidação dos mecanismos de determinação sexual nos peixes:

contribuições obtidas através do mapeamento físico cromossômico e caracterização

nucleotídica de genes envolvidos na determinação e diferenciação sexual em peixes

ciclídeos. Financiamento FAPESP 2009/05234-4. Projeto em andamento.

Vigência 01/08/2009 a 31/07/2011

Total de Recursos Aprovados: R$223.017,04; US$29.107,29

Parcela Reserva Técnica Departamental: R$ 32.784,52

Projeto 2: Análise comparativa de genes candidatos à determinação e diferenciação

sexual em vertebrados utilizando os peixes Cichla monoculus e Danio rerio como

organismos modelo. Financiamento CNPq 475147/2010-3. Projeto em andamento.

Vigência: Dez/2010-Nov/2012.

Total de Recursos Aprovados: R$20.000,00

6- Lista das publicações resultantes do auxílio no período.

Artigos em revistas científicas indexadas;

Poletto AB, Ferreira IA, Martins C (2010). The B chromosome of the cichlid fish

Haplochromis obliquidens harbors 18S rRNA genes. BMC Genetics 11: 1.

! "##!

Toniato J, Penman DJ, Martins C (2010). Discrimination of tilapia species of the genera

Oreochromis, Tilapia and Sarotherodon by PCR-RFLP of 5S rDNA. Aquaculture

Research 41: 934-938.

Gross MC, Schneider CH, Valente GT, Martins C, Feldberg E (2010). Variability inter

and intraspecific of 18S rDNA locus among Symphysodon fishes: chromosomal

rearrangements. Journal of Fish Biology 76: 1117-1127.

Cioffi MB, Martins C, Vicari MR, Rebordinos L, Bertollo LAC (2010). Differentiation

of the XY sex chromosomes in Hoplias malabaricus (Characiformes, Erythrinidae).

Unusual accumulation of repetitive sequences on the X chromosome. Sexual

Development 4 (3): 176-185.

Poletto AB, Ferreira IA, Cabral-de-Mello DC, Nakajima R, Mazzuchelli J, Ribeiro HB,

Venere PC, Nirchio M, Kocher TD, Martins C (2010). Chromosome differentiation

patterns during cichlid fish evolution. BMC Genetics 11:50.

Mota-Velasco JC, Ferreira IA, Cioffi MB, Ocalewicz K, Campos-Ramos R, Shirak A,

Lee B-Y, Martins C, Penman DJ (2010). Characterization of the chromosome

fusions in Oreochromis karongae. Chromosome Research 18:575–586.

Valente GT, Mazzuchelli J, Ferreira IA, Poletto A, Martins C (2011). Cytogenetic

mapping of the retroelements Rex1, Rex3 and Rex6 among cichlid fish: new insights

on the chromosomal distribution of transposable elements. Cytogenetic and Genome

Research 133(1): 34-42.

Vitorino CA, Souza IL, Rosa JN, Valente GT, Martins C, Venere PC (2011). Molecular

cytogenetics and its contribution to the understanding of the chromosomal

diversification in Hoplias malabaricus (Characiformes). Journal of Fish Biology

78(4): 1239-1248.

Pinhal D, Yoshimura TS, Araki CS, Martins C (2011). The 5S rDNA family evolves

through concerted and birth-and-death evolution in fish genomes: an example from

freshwater stingrays. BMC Evolutionary Biology 11: 151.

Mazzuchelli J, Yang F, Kocher TD, Martins C (2011). Comparative cytogenetic

mapping of Sox2 and Sox14 in cichlid fishes and inferences on the genomic

organization of both genes in vertebrates. Chromosome Research 19(5): 657-667.

Artigos em fase final de redação e submetidos

Mazzuchelli J, Yang F, Kocher K, Martins C. Genome stability in cichlid fish:

molecular cytogenetic and genomic approaches. BMC Genomics.

! "#%!

Cabral-de-Mello DC, Nakajima RT, Martins C. Genomic organization and comparative

chromosome mapping of U1 snRNA gene in cichlid fish, with emphasis in

Oreochromis niloticus. Possível submissão à revista Chromosome Research.

Nakajima RT, Cabral-de-Mello DC, Martins C. Dynamics of 5S rDNA clusters during

karyotype diversification in cichlid fish. Possível submissão à revista Cytogenetic

and Genome Research.

Artigos em fase inicial de redação

Characterization and evolution of nucleotide sequences and protein domain structure of Foxl2 gene: insights through vertebrates. Marcos Tadeu Geraldo, Guilherme Targino Valente and Cesar Martins

Introducing Chondrichthyes species in the evolutionary history of Sox9 gene. Marcos Tadeu Geraldo, Guilherme Targino Valente and Cesar Martins

Identification and characterization of two sex determining genes in the Neotropical cichlid Cichla monoculus focusing in comparative analysis for vertebrates. Guilherme Targino Valente, Marcos Tadeu Geraldo and Cesar Martins

Analysis of promoter prediction of Sox9, Foxl2, Cyp19a and Dmrta2 genes. Building a brief evolutionary history of their putative expression regulation. Guilherme Targino Valente, Marcos Tadeu Geraldo and Cesar Martins

In silico mapping of vertebrate sex determination and differentiation genes in Oreochromis niloticus genome. Checking old hypothesis in a recently sequenced genome. Guilherme Targino Valente, Marcos Tadeu Geraldo, Rafael Takahiro Nakajima, Thomas Kocher and Cesar Martins

Universal protein-protein prediction (UP3i) based on protein physical-chemical features. Guilherme Targino Valente, Marcio Luis Acencio, Ney Lemke and Cesar Martins

Network prediction of protein-protein interactions based on UP3i for zebrafish and Nile tilapia genomes: Sub-network analysis of sex determination and differentiation pathways. Guilherme Targino Valente, Marcio Luis Acencio, Ney Lemke, Thomas Kocher and Cesar Martins

Capítulos de livros publicados;

Cabral-de-Mello DC, Martins C (2010). Breaking down the genome organization and

karyotype differentiation through the epifluorescence microscope lens: insects and

fishes as models. In Microscopy: Science, Technology, Application and Education.

! "#&!

Formatex Microscopy No. 4, Vol. 1. A Méndez-Vilas; J Díaz. (Eds.). Formatex

Research Center, Badajoz, Spain. pp 658-669.

Martins C, Cabral-de-Mello DC, Valente GT, Mazzuchelli J, Oliveira SG (2011).

Cytogenetic mapping and its contribution to the knowledge of animal genomes. In

Advances in Genetics Research, Vol. 4 Kevin V Urbano (Ed.). Nova Science

Publisher, Hauppauge, NY, USA. Pp. 1-82.

Martins C, Vicari MR (2011). Hibridação in situ em cromossomos de peixes. In:

Citogenética Molecular: Protocolos Comentados. Ed Guerra M, Editora SBG,

Ribeirão Preto. no prelo.

Livros publicados com membros da equipe como autor, organizador ou editor;

Costa AM, Martins C (2010). Estrutura e evolução de genomas. Editora Emprapa.

Planaltina, DF. 110 pp.

Martins C, Cabral-de-Mello DC, Valente GT, Mazzuchelli J, Oliveira SG, Pinhal D

(2011). Animal genomes under the focus of cytogenetics. Nova Science Publisher,

Hauppauge, NY, USA. No prelo.

Dissertações defendidas;

Bruno Evaristo de Almeida Fantinatti, Bolsista FAPESP

Programa de Pós-Graduação em Ciências Biológicas (Genética), IB/UNESP,

Botucatu, SP. Contribuições ao conhecimento de cromossomos supernumerários em

peixes, utilizando como modelo Haplochromis obliquidens (Perciformes, Cichlidae)

2009-2011.

Teses defendidas;

Irani Alves Ferreira, Bolsista FAPESP

Programa de Pós-Graduação em Ciências Biológicas (Genética),

IB/UNESP, Botucatu, SP.

Projeto “Mapeamento cromossômico comparativo em peixes ciclídeos

utilizando seqüências repetitivas de DNA”. 2005-2009.

Andréia B Poletto, Bolsista CAPES

Programa de Pós-Graduação em Ciências Biológicas (Genética),

IB/UNESP, Botucatu, SP.

Projeto “Citogenética comparativa de peixes da família Cichlidae”. 2005-2009.

! "#'!

Maria Cláudia Gross, Bolsista CNPq

Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva

(INPA). Projeto “Citogenética comparativa das variedades selvagens de acará-disco

(Symphysodon spp., Cichlidae, Perciformes) endêmicos da Amazônia: uma

abordagem clássica e molecular dos cromossomos mitóticos e meióticos”. 2006-

2009.

Teses e Dissertações em andamento;

Marcos Tadeu Geraldo (Mestrado), Bolsista FAPESP

Programa de Pós-Graduação em Ciências Biológicas (Genética),

IB/UNESP, Botucatu, SP.

Projeto “Determinação e diferenciação sexual em vertebrados basais:

análise comparativa de genes candidatos em Agnatha e Chondrichthyes”.

2010-2012.

Guilherme Targino Valente (Doutorado), Bolsista FAPESP

Programa de Pós-Graduação em Ciências Biológicas (Genética),

IB/UNESP, Botucatu, SP.

Projeto “Análise comparativa de genes candidatos à determinação e

diferenciação sexual em vertebrados utilizando ciclídeos sul-americanos

como organismos modelo”. 2009-2013.

Atividades de Extensão;

O desenvolvimento do projeto possibilitou que o Laboratório Genômica Integrativa

organizasse cursos de curta duração, fornecendo aprendizado e treinamento em novas

tecnologias de análise genômica. Além disso, o envolvimento do grupo foi também efetivo em

projetos de extensão voltados a difusão e popularização da ciência.

Cursos organizados

“Curso de extensão universitária: VI Curso de Verão Manipulação de ácidos nucléicos: do DNA

ao produto gênico”, 09 a 13 de fevereiro de 2009, IBB/UNESP-Botucatu.

“Introdução pratica a biologia molecular”. Curso de extensão de 40 horas ministrado de

26 a 30 de julho de 2010 no Instituto de Biociências da UNESP, Campus de

Botucatu, SP.

! "%(!

“Avanços em biologia molecular”. Curso de extensão de 40 horas ministrado de 18 a 22

de julho de 2011 no Instituto de Biociências da UNESP, Campus de Botucatu, SP.

Programas de extensão

Projeto de Extensão “Difundindo e Popularizando a Ciência”. 2009 a 2011, IB/UNESP-

Botucatu. Projeto que atende estudantes e professores do ensino médio, oferecendo oficinas

e atividades inovadoras voltadas ao ensino de ciências.

! "%"!

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